White-nose syndrome is an emerging disease in Europe and North America, causing significant decline in bat populations. This study focused on bacteria associated with white-nose syndrome infected bats. Eighty-two microorganisms were grown in pure culture; fourteen of these have been identified. No significant differences were found between the microbiota of fluorescing and nonfluorescing bats. P. fluorescens along with other Pseudomonas species could be responsible for the fluorescence of the cultured microbes.